TIFF data conversion -------------------- Install NeuroConv with the additional dependencies necessary for reading TIFF data. .. code-block:: bash pip install "neuroconv[tiff]" Convert TIFF imaging data to NWB using :py:class:`~neuroconv.datainterfaces.ophys.tiff.tiffdatainterface.TiffImagingInterface`. .. code-block:: python >>> from datetime import datetime >>> from zoneinfo import ZoneInfo >>> from pathlib import Path >>> from neuroconv.datainterfaces import TiffImagingInterface >>> >>> file_path = OPHYS_DATA_PATH / "imaging_datasets" / "Tif" / "demoMovie.tif" >>> interface = TiffImagingInterface(file_path=file_path, sampling_frequency=15.0, verbose=False) >>> >>> metadata = interface.get_metadata() >>> # For data provenance we add the time zone information to the conversion >>> session_start_time = datetime(2020, 1, 1, 12, 30, 0, tzinfo=ZoneInfo("US/Pacific")) >>> metadata["NWBFile"].update(session_start_time=session_start_time) >>> >>> # Choose a path for saving the nwb file and run the conversion >>> nwbfile_path = f"{path_to_save_nwbfile}" >>> interface.run_conversion(nwbfile_path=nwbfile_path, metadata=metadata) .. note:: The :py:class:`~neuroconv.datainterfaces.ophys.tiff.tiffdatainterface.TiffImagingInterface` is designed for imaging data where all of the frames are in a multi-page TIFF file. It is not appropriate for datasets where the TIFF data is distributed across many files, for example from Bruker acquisition software.